Journal: PLoS ONE
Article Title: Edesign: Primer and Enhanced Internal Probe Design Tool for Quantitative PCR Experiments and Genotyping Assays
doi: 10.1371/journal.pone.0146950
Figure Lengend Snippet: (A-D) Evaluation for mutation detection using pandemic 2009 H1N1 influenza virus genome segment 6 (neuraminidase) H275Y mutation; (E-H) Evaluation for low copy DNA detection using influenza B virus genome segment 7 (matrix protein) as a template. Upper panels (A, C, E, G) show results obtained by using Eprobes and primers designed by Primer3 with updated tables for T M calculation of ECHOs. Lower panels (B, D, F, H) show results obtained by using Eprobes and primers designed by Edesign. Details on primers and Eprobes used are provided in and Figs.
Article Snippet: Edesign is based on the Primer3 software (Primer3 core C program version 2.3.4 and Primer3 web interface version 3.0.0), for which the source code was downloaded at http://sourceforge.net/projects/primer3/files/primer3/ , and for the web interface at http://sourceforge.net/projects/primer3/files/primer3-web/ .
Techniques: Mutagenesis, Virus